Analysis of Article on Aeromonas veronii and its Adaptation to the Zebrafish Gut

Analysis of Article on Aeromonas veronii and its Adaptation to the Zebrafish Gut

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Analysis of Article on Aeromonas veronii and its Adaptation to the Zebrafish Gut

Aeromonas veronii is a Gram-negative bacterium that has the shape of a rod surviving in fresh water and is associated with animals. It can harm humans and plays a beneficial role in leeches. In humans, it causes diseases characterized by wounds, diarrhea, and sepsis in immunocompromised patients. The major disease associated with the bacterium is gastroenteritis, which occurs after consuming contaminated food and water. The organism expresses itself in different traits. In vertebrates, the screening of genome has been used by in the scientific study of living organism and their adaptation to Aeromonas veronii infections (Robinson et al., 2018). As such, the traits have been examined through Vivo experiments to determine the bacterium colonization. It colonizes the animal tissues lie the zebrafish. As such, the research uses the zebrafish as an animal host model in investigating how the bacterium increases its ability to become host-associated. The researcher, therefore, involved exposing the zebra host to bacterium through the provision of the primary election of the host association (Robinson et al., 2018). The research uses the powerful model system to determine the evolution by passing the bacterium through GF larval zebrafish host by using a gut-associated population as inoculum for subsequent passage. As a result, the host adaptation occurred so quick across the replicate line in production. The phenotypic characteristics of adaptation showed no specific features with the host environment. Further adaptation confirms the specialization of the host through the evolution into different genotypes.

The analysis of the colonization cycle of the Aeromonas veronii involves incubating the zebrafish host in the water Colum by involving the embryo medium. Also, the experiment in the study dogged the toughness of Aeromonas veronii colonization as a purpose of inoculum absorption which can vary during the ovulation model. The importance of the divisional colonization of the organization gets based on influencing the genetic variant that enhances sampling within the model system. Consequently, the additional colonization of Aeromonas veronii aided in investigating colonization success (Robinson et al., 2018). The article describes how the experiment identified the strains that promoted the host association through the replicate clonal mutation of Aeromonas veronii. As such, the organism was incubated in a flask containing the larval fish of the external medium in the system. After three days, the guts of the fish were harvested in the experiment through dissection, which involved the association of the Aeromonas veronii population.

Further determination of the initial adaptation of Aeromonas veronii created the observation of four isolates in the improved fitness that instigated the stages of the colonization cycle. Confirmation of the cycle entailed isolation that distinguished fitness specific to the environment. The findings depict that immigration effects on several facets of the colonization changing aspects through the host-to-host transmission because it has an abundance nature in the emigration cycle. Notably, the enhanced spread is projected to alter several important aspects of the host microbes’ ecology. The population statistics, according to the data generated by the experiment, confirms that the bacterial growth and death plus their expulsion from the gut are stochastic. Moreover, the bacterial traits show the mortification that is adaptable to the trait for the host colonization. The evolved isolates contain the hypermobile phenotype as compared to the ancestor organism. However, the host phenotype never promoted competitive fitness (Robinson et al., 2018). The identification of the bacterial trait conferred the host association through the phenotypic characteristics of the already evolved isolates. Determining the genetic basics of the adaptive features provided a deeper description of the biological mechanism in the zebrafish. Therefore, the increased number of mutator strain is capable of making the adaptive mutation.

Reference

Robinson, C. D., Klein, H. S., Murphy, K. D., Parthasarathy, R., Guillemin, K., & Bohannan, B. J. (2018). Experimental bacterial adaptation to the zebrafish gut reveals a primary role in immigration. PLoS biology, 16(12), e2006893. https://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.2006893